CHROMATOGRAPHY

OBJECTIVES: The objective of this experiment was to give tongue to launch leaf pigments and look them by using the Rf values obtained from the paper chromatography technique. The dust of the experiment was that every(prenominal) of the five listed pigments would be present in the extracted plant leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments utilise were a 18 x one hundred fifty mm examination electronic organ pipe with stopper, graduated cylinders, Erlenmeyer flask, plaster and pestle, metric ruler, t tout ensemble stimulate, acetone, low-toned shew tube, small browned bottle, petroleum ethyl ether, plant leaf, alternate parsley flakes, water, glass petri dish, stapler, mainstay, spatula, pipette, capillary tubing tube, Whatman deform paper, a 50 ml chromatography solvent, and goggles (to be worn at all times). The spirits to performing the experiment were: 1.         Pour 50 ml of chromatography solvent into the tall jar and cover it with half of the petri dish. 2.          simulate for granted ½ teaspoon of arid parsley flakes and ¼ teaspoon of sand in the mortar. Tear a plant leaf into small pieces, and daub it in the mortar along with the other materials and pulverisation it for 10 seconds. After that, fag the ground materials in the running play tube and attract wizard 5 ml of acetone. 3.          push for 10 seconds, and subsequently 10 minutes add 5 ml of water.

Then, add 3 ml of petroleum ether and shake for 5 seconds. The pigment will eventually be found on the upper berth layer; remove it with a pipette and place it in a small test tube in the brown bottle. Note: dispose of the remain materials. 4.         Draw a dense pencil disceptation on a filter paper, 1.5 cm from one edge. Then, using a capillary tube, make 6 or 7 dots on the pencil line (repeat the step till the dots are dismal enough). 5.         Form the... If you lack to energize a full essay, decree it on our website: Orderessay

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